Phospho-ILK-1 (Thr173) Polyclonal Antibody, AbBy Fluor™ 750 Conjugated

Product Specifications

Background

Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.

Synonyms

P59; ILK-1; ILK-2; p59ILK; HEL-S-28; Integrin-linked protein kinase; 59 kDa serine/threonine-protein kinase; ILK; ILK1; ILK2

Gene ID

3611

Swiss Prot

Q13418

Modification Site

Thr173

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Phospho-ILK-1 (Thr173)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 750

Source

KLH conjugated synthetic phosphopeptide derived from human ILK-1 around the phosphorylation site of Thr173

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

749nm/775nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

3611

Predicted Cross Reactivity

Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit

Curated Selection

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