Phospho-CK18 (Ser60) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated
Product Specifications
Background
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
Synonyms
Cytokeratin 18 phospho S60; p-Cytokeratin 18 phospho S60; Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; Cell proliferation-inducing gene 46 protein; CK 18; CK-18; CK18; CYK 18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin-18; Cytokeratin18; K 18; K18; K1C18_HUMAN; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; keratin, type I cytoskeletal 18; Keratin-18; Keratin18; Kerd; KRT18.
Gene ID
3875
Modification Site
Ser60
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Target
Phospho-CK18 (Ser60)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 488
Source
KLH conjugated synthetic phosphopeptide derived from human CK18 around the phosphorylation site of Ser60 [MG (p-S) GG]
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
499nm/519nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
3875
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