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Phospho-G3BP1 (Ser232) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated

Product Specifications

Background

May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.

Synonyms

G3BP; HDH-VIII; Ras GTPase-activating protein-binding protein 1; G3BP-1; ATP-dependent DNA helicase VIII; hDH VIII; GAP SH3 domain-binding protein 1; G3BP1

Gene ID

10146

Swiss Prot

Q13283

Modification Site

Ser232

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-G3BP1 (Ser232)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 488

Source

KLH conjugated synthetic phosphopeptide derived from human G3BP1 around the phosphorylation site of Ser232

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

499nm/519nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-5376R-BF488

Gene ID URL

10146

Predicted Cross Reactivity

Cow, Pig, Rabbit

Curated Selection

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