Phospho-GIT1 (Tyr510) Polyclonal Antibody, AbBy Fluor™ 405 Conjugated

Product Specifications

Background

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity) .

Synonyms

ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; GIT1

Gene ID

28964

Swiss Prot

Q9Y2X7

Modification Site

Tyr510

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-GIT1 (Tyr510)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 405

Source

KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr510

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

401nm/421nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

28964

Predicted Cross Reactivity

Human, Rat, Cow, Pig, Chicken

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