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Phospho-EIF4B (Ser504) Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot) .

Synonyms

E 4B; E-4B; Eukaryotic initiation factor 4B; 4B_HUMAN.

Gene ID

1975

Modification Site

Ser504

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-EIF4B (Ser504)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human EIF4B around the phosphorylation site of Ser504

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1975

Predicted Cross Reactivity

Human, Dog, Cow, Horse, Rabbit

Curated Selection

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