Phospho-EIF4B (Ser504) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot) .
Synonyms
E 4B; E-4B; Eukaryotic initiation factor 4B; 4B_HUMAN.
Gene ID
1975
Modification Site
Ser504
Cellular Locus
Cytoplasm
Host
Rabbit
Target
Phospho-EIF4B (Ser504)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human EIF4B around the phosphorylation site of Ser504
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1975
Predicted Cross Reactivity
Human, Dog, Cow, Horse, Rabbit
Curated Selection
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