Phospho-EIF4B (Ser504) Polyclonal Antibody, AbBy Fluor™ 488 Conjugated
Product Specifications
Background
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot) .
Synonyms
E 4B; E-4B; Eukaryotic initiation factor 4B; 4B_HUMAN.
Gene ID
1975
Modification Site
Ser504
Cellular Locus
Cytoplasm
Host
Rabbit
Target
Phospho-EIF4B (Ser504)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 488
Source
KLH conjugated synthetic phosphopeptide derived from human EIF4B around the phosphorylation site of Ser504
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
499nm/519nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1975
Predicted Cross Reactivity
Human, Dog, Cow, Horse, Rabbit
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