Phospho-EIF4B (Ser504) Polyclonal Antibody, APC-Cy7 Conjugated

Product Specifications

Background

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot) .

Synonyms

E 4B; E-4B; Eukaryotic initiation factor 4B; 4B_HUMAN.

Gene ID

1975

Modification Site

Ser504

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-EIF4B (Ser504)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy7

Source

KLH conjugated synthetic phosphopeptide derived from human EIF4B around the phosphorylation site of Ser504

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/780nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1975

Predicted Cross Reactivity

Human, Dog, Cow, Horse, Rabbit

Curated Selection

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