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Phospho-ECT2 (Thr373) Polyclonal Antibody, Cy5.5 Conjugated

Product Specifications

Background

The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq].

Synonyms

ECT2 phospho T373; ECT2 phospho Thr373; p-ECT2 Thr373; ECT 2; ECT2; ECT-2; ECT2 protein; Epithelial cell transforming sequence 2; Epithelial cell transforming sequence 2 oncogene; Epithelial cell transforming sequence 2 oncogene protein; FLJ10461; MGC138291; Protein ECT2; ECT2_HUMAN; Epithelial cell-transforming sequence 2 oncogene.

Gene ID

1894

Modification Site

Thr373

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Target

Phospho-ECT2 (Thr373)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

675nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

1894

Predicted Cross Reactivity

Human, Dog, Cow, Sheep

Curated Selection

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