Phospho-EP300 (Ser1834) Polyclonal Antibody, PerCP-Cy5.5 Conjugated

Product Specifications

Background

KAT3B/p300 is a transcriptional adapter protein (300 kDa) which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP (for CREB-binding protein) . KAT3B and KAT3A are homologous to each other.

Synonyms

CREBBP/EP300 inhibitory protein 1; Cyclic AMP responsive enhancer binding protein; E1A associated protein p300; E1A binding protein p300; EC 2.3.1.48; EP300; EP300: E1A binding protein p300; RB and P300 binding protein EID 1; Histone acetyltransferase p300; p300 HAT; Retinoblastoma protein associated protein.

Gene ID

2033

Modification Site

Ser1834

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-EP300 (Ser1834)

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP-Cy5.5

Source

KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/694nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

2033

Predicted Cross Reactivity

Rat, Dog, Cow, Pig, Horse, Chicken, Guinea Pig

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