H3K27me3 Polyclonal Antibody
Product Specifications
Background
A core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene ID
8350
Swiss Prot
P68431
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat, Others, Zebrafish, Drosophila
Target
H3K27me3
Clonality
Polyclonal
Conjugation
Unconjugated
Source
Polyclonal antibody raised in rabbit against against histone H3, trimethylated at lysine 27 (H3K27me3), using a KLH-conjugated synthetic peptide
Applications
WB, ELISA, IF, dot-blot, ChIP-seq
Purification
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Concentration
1.45µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IF (), dot-blot (), ChIP-seq ()
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
8350
Cross Reactivity Details
Schistosoma, Arabidopsis
Curated Selection
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