H4K20me3 Polyclonal Antibody
Product Specifications
Background
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene ID
121504
Swiss Prot
P62805
Host
Rabbit
Cross Reactivity
Human, Mouse, Others
Target
H4K20me3
Clonality
Polyclonal
Conjugation
Unconjugated
Source
Polyclonal antibody raised in rabbit against the region of histone H4 containing the trimethylated lysine 20 (H4K20me3), using a KLH-conjugated synthetic peptide
Applications
WB, ELISA, IHC-P, IF (ICC), IF, dot-blot, ChIP-seq
Purification
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Concentration
1.75µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IF (ICC) (1:50-200), IF (), dot-blot (), ChIP-seq ()
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
121504
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