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H3K4me1 Polyclonal Antibody

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

8350

Swiss Prot

P68431

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

H3K4me1

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Polyclonal antibody raised in rabbit against histone H3 containing the monomethylated lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide

Applications

WB, ELISA, IF, dot-blot, ChIP-seq

Purification

Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

3µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IF (), dot-blot (), ChIP-seq ()

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C for 12 months.

Gene ID URL

8350

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