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H3K9/14ac Polyclonal Antibody

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

8350

Swiss Prot

P68431

Host

Rabbit

Cross Reactivity

Human, Mouse, Others

Target

H3K9/14ac

Clonality

Polyclonal

Conjugation

Unconjugated

Source

Polyclonal antibody raised in rabbit against histone H3 acetylated at lysines 9 and 14 (H3K9/14ac), using a KLH-conjugated synthetic peptide

Applications

WB, ELISA, IF, dot-blot, ChIP-seq

Purification

Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.

Concentration

1.4µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IF (), dot-blot (), ChIP-seq ()

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C for 12 months.

Gene ID URL

8350

Cross Reactivity Details

A. Nidulans, Arabidopsis

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