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Phospho-CDK1 (Ser39) Polyclonal Antibody, Cy5 Conjugated

Product Specifications

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

P34CDC2; Cdc 2; CDK 1; CDK1; Cell division control protein 2; Cell division control protein 2 homolog; Cell division cycle 2 G1 to S and G2 to M; Cell Divsion Cycle 2 Protein; CDK1_HUMAN.

Gene ID

983

Modification Site

Ser39

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-CDK1 (Ser39)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy5

Source

KLH conjugated synthetic phosphopeptide derived from human cdc2 around the phosphorylation site of Ser39 [LE (p-S) EE]

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

625,650nm/670nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

983

Predicted Cross Reactivity

Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit

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