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Phospho-Cdc25B (Ser353) Polyclonal Antibody, RBITC Conjugated

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Synonyms

M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2

Gene ID

994

Swiss Prot

P30305

Modification Site

Ser353

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-Cdc25B (Ser353)

Clonality

Polyclonal

Isotype

IgG

Conjugation

RBITC

Source

KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353

Applications

WB

Purification

Purified by Protein A.

Excitation Emission

570nm/595nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.biossantibodies.com/datasheets/bs-5245R-RBITC

Specificity

This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat.

Gene ID URL

994

Predicted Cross Reactivity

Human, Rat

Curated Selection

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