Phospho-Cdc25B (Ser353) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated
Product Specifications
Background
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Synonyms
M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Gene ID
994
Swiss Prot
P30305
Modification Site
Ser353
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-Cdc25B (Ser353)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 647
Source
KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser353
Applications
WB
Purification
Purified by Protein A.
Excitation Emission
650nm/665nm
Concentration
1µg/µl
Dilution
WB (1:300-5000)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This phosphorylation site is homologous to that of Ser351 in Mouse and Ser 349 in Rat.
Gene ID URL
994
Predicted Cross Reactivity
Human, Rat
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