Phospho-Cdc25B (Ser186) Polyclonal Antibody, AbBy Fluor™ 680 Conjugated
Product Specifications
Background
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Synonyms
M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2
Gene ID
994
Swiss Prot
P30305
Modification Site
Ser186
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-Cdc25B (Ser186)
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 680
Source
KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser186
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
679nm/702nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
994
Predicted Cross Reactivity
Human, Rat
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS1186690-01 1 mg (E-Coli)
Recombinant Escherichia coli Tellurite resistance protein tehA (tehA), partial
Ask
View Details MBS1186690-02 1 mg (Yeast)
Recombinant Escherichia coli Tellurite resistance protein tehA (tehA), partial
Ask
View Details 
MBS1076234-01 0.02 mg (E-Coli)
Recombinant Bacillus subtilis Uncharacterized protein yeaD (yeaD)
Ask
View Details 
MBS1076234-02 0.02 mg (Yeast)
Recombinant Bacillus subtilis Uncharacterized protein yeaD (yeaD)
Ask
View Details MBS1076234-03 0.1 mg (E-Coli)
Recombinant Bacillus subtilis Uncharacterized protein yeaD (yeaD)
Ask
View Details