Phospho-Cdc25B (Ser323) Polyclonal Antibody, PE-Cy7 Conjugated

Product Specifications

Background

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Synonyms

M-phase inducer phosphatase 2; Dual specificity phosphatase Cdc25B; CDC25B; CDC25HU2

Gene ID

994

Swiss Prot

P30305

Modification Site

Ser323

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-Cdc25B (Ser323)

Clonality

Polyclonal

Isotype

IgG

Conjugation

PE-Cy7

Source

KLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser323

Applications

Purification

Purified by Protein A.

Excitation Emission

488nm/780nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

994

Predicted Cross Reactivity

Human, Mouse, Rat

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