Phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, AbBy Fluor™ 405 Conjugated

Product Specifications

Background

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Synonyms

P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; CDKN1A; MDA6; PIC1

Gene ID

1026

Swiss Prot

P38936

Modification Site

Thr145

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-CDKN1A/p21 (Thr145)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 405

Source

KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr145 [RQ (p-T) S]

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

401nm/421nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous to that of Thr140 in Mouse and Thr145 is Rat.

Gene ID URL

1026

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

MBS2806044-01 48 Tests

Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

Ask

View Details

MBS2806044-02 96 Tests

Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

Ask

View Details

MBS2806044-03 5x 96 Tests

Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

Ask

View Details

MBS2806044-04 10x 96 Tests

Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) ELISA Kit

Ask

View Details

abx968139-01 150 µg

Human C9orf152 shRNA Plasmid

Ask

View Details

abx968139-02 300 µg

Human C9orf152 shRNA Plasmid

Ask

View Details