Phospho-CDKN1A/p21 (Thr145) Polyclonal Antibody, APC Conjugated

Product Specifications

Background

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

Synonyms

P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; CDKN1A; MDA6; PIC1

Gene ID

1026

Swiss Prot

P38936

Modification Site

Thr145

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-CDKN1A/p21 (Thr145)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC

Source

KLH conjugated synthetic phosphopeptide derived from human CDKN1A around the phosphorylation site of Thr145 [RQ (p-T) S]

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/660nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous to that of Thr140 in Mouse and Thr145 is Rat.

Gene ID URL

1026

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