Phospho-ATG3 (Tyr18) Polyclonal Antibody, Cy5.5 Conjugated
Product Specifications
Background
E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE) . PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.
Synonyms
2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; APG3-like; APG3L; Apg3p; ATG 3; ATG3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog S. cerevisiae; ATG3_HUMAN; Autophagy Apg3p/Aut1p like; Autophagy related protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3.
Gene ID
64422
Modification Site
Tyr18
Cellular Locus
Cytoplasm
Host
Rabbit
Target
Phospho-ATG3 (Tyr18)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human ATG3 around the phosphorylation site of Tyr18
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
675nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
64422
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS6356546-01 0.1 mL
TMIE, ID (TMIE, Transmembrane inner ear expressed protein) (MaxLight 750)
Ask
View Details MBS6356546-02 5x 0.1 mL
TMIE, ID (TMIE, Transmembrane inner ear expressed protein) (MaxLight 750)
Ask
View Details