Phospho-ATG3 (Tyr18) Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes the conjugation of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to phosphatidylethanolamine (PE) . PE-conjugation to ATG8-like proteins is essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3.

Synonyms

2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; APG3-like; APG3L; Apg3p; ATG 3; ATG3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog S. cerevisiae; ATG3_HUMAN; Autophagy Apg3p/Aut1p like; Autophagy related protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3.

Gene ID

64422

Modification Site

Tyr18

Cellular Locus

Cytoplasm

Host

Rabbit

Target

Phospho-ATG3 (Tyr18)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic phosphopeptide derived from human ATG3 around the phosphorylation site of Tyr18

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

64422

Predicted Cross Reactivity

Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

160683 1 mg

3-O-Methyl-L-DOPA 4-Glucuronide

Ask

View Details

BM4094B 200 µg

MHC Class II (I-A k,b,d,q,r) Rat Monoclonal Antibody [Clone ID: ER-TR3]

Ask

View Details

ES6398-01 50 µL

NOS2 (phospho Tyr151) rabbit pAb

Ask

View Details

ES6398-02 100 µL

NOS2 (phospho Tyr151) rabbit pAb

Ask

View Details

MBS6455737-01 0.2 mL

EHD4 (EH Domain-Containing Protein 4, Hepatocellular Carcinoma-Associated Protein 10/11, PAST Homolog 4, EHD4, HCA10, HCA11, PAST4) (HRP)

Ask

View Details

MBS6455737-02 5x 0.2 mL

EHD4 (EH Domain-Containing Protein 4, Hepatocellular Carcinoma-Associated Protein 10/11, PAST Homolog 4, EHD4, HCA10, HCA11, PAST4) (HRP)

Ask

View Details