Phospho-ATG9A (Ser735) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.
Synonyms
MATG9; APG9L1; MGD3208; Autophagy-related protein 9A; APG9-like 1; ATG9A
Gene ID
79065
Swiss Prot
Q7Z3C6
Modification Site
Ser735
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Target
Phospho-ATG9A (Ser735)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human ATG9A around the phosphorylation site of Ser735
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
79065
Predicted Cross Reactivity
Human, Mouse, Rat, Cow, Pig
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