Phospho-ATG9A (Ser735) Polyclonal Antibody, AbBy Fluor™ 405 Conjugated

Product Specifications

Background

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Synonyms

MATG9; APG9L1; MGD3208; Autophagy-related protein 9A; APG9-like 1; ATG9A

Gene ID

79065

Swiss Prot

Q7Z3C6

Modification Site

Ser735

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Target

Phospho-ATG9A (Ser735)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 405

Source

KLH conjugated synthetic phosphopeptide derived from human ATG9A around the phosphorylation site of Ser735

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

401nm/421nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

79065

Predicted Cross Reactivity

Human, Mouse, Rat, Cow, Pig

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