EIF4E Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Product Specifications
Background
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Synonyms
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
Gene ID
1977
Swiss Prot
P06730
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat
Immunogen
121-217/217
Target
EIF4E
Clonality
Polyclonal
Isotype
IgG
Conjugation
PerCP-Cy5.5
Source
KLH conjugated synthetic peptide derived from human eIF4E
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
482nm/694nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1977
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