Hepatitis C Virus RNA-directed RNA polymerase Antibody, AbBy Fluor™ 350 Conjugated
Selected Variant
bs-4858R-BF350
Size: 100 µL
Product Specifications
Background
The RNA directed RNA polymerase is also known as non-structural protein NS5B. NS5B is a 65 kDa protein that resembles other viral RNA polymerases. Hepatitis C virus (HCV) replication is thought to occur in membrane bound replication complexes. These complexes transcribe the positive strand and the resulting minus strand is used as a template for the synthesis of genomic RNA. There are two viral proteins involved in the reaction, NS3 and NS5B
Synonyms
RNA-directed RNA polymerase; p68; RNA dependent RNA polymerase.
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Virus
Immunogen
2501-2600/3010
Target
Hepatitis C Virus RNA-directed RNA polymerase
Clonality
Polyclonal
Isotype
IgG
Conjugation
AbBy Fluor™ 350
Source
KLH conjugated synthetic peptide derived from Hepatitis C Virus RNA-directed RNA polymerase
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
346nm/442nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Cross Reactivity Details
HCV
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