HCV NS4B Polyclonal Antibody, PE Conjugated
Product Specifications
Background
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. NS4B induces a specific membrane alteration that serves as a scaffold for the virus replication complex. This membrane alteration gives rise to the so-called ER-derived membranous web that contains the replication complex.
Synonyms
HCV; HCV NS4B; Non structural protein 4B; NS4B; p27.
Swiss Prot
P26663
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Virus
Immunogen
1712-1820/3010
Target
HCV NS4B
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE
Source
KLH conjugated synthetic peptide derived from Hepatitis C Virus NS4B
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
496,564nm/578nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Cross Reactivity Details
HCV
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