Bcl-2 Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Product Specifications

Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) .

Synonyms

Bcl-2; PPP1R5; Apoptosis regulator Bcl-2; BCL2

Gene ID

596

Swiss Prot

P10415

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Immunogen

161-239/239

Target

Bcl-2

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic peptide derived from human Bcl-2

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

596

Available Sizes

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