SPARC Polyclonal Antibody, Biotin Conjugated
Product Specifications
Background
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.
Synonyms
AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin) ; Secreted protein acidic cysteine rich; SPRC; SPRC_ .
Gene ID
6678
Swiss Prot
P09486
Cellular Locus
Extracellular, Secreted, Cell membrane
Host
Rabbit
Cross Reactivity
Mouse, Rat
Target
SPARC
Clonality
Polyclonal
Isotype
IgG
Conjugation
Biotin
Source
Recombinant SPARC protein
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Affinity purified by Protein A
Concentration
1mg/ml
Dilution
WB (WB=1:500-2000), ELISA (ELISA=1:5000-10000), IHC-P (IHC-P=1:400-800), IHC-F (IHC-F=1:400-800)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C for 12 months.
Gene ID URL
6678
Predicted Cross Reactivity
Human
Curated Selection
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