SPARC Polyclonal Antibody

Product Specifications

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca (2+) with a low affinity and an EF-hand loop that binds a Ca (2+) ion with a high affinity.

Synonyms

AA517111; Basement membrane protein 40; BM 40; BM40; Cysteine rich protein; hm:zeh0062; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin) ; Secreted protein acidic cysteine rich; SPRC; SPRC_ .

Gene ID

6678

Swiss Prot

P09486

Cellular Locus

Extracellular, Secreted, Cell membrane

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

SPARC

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

Recombinant SPARC protein

Applications

WB, ELISA, IHC-P, IHC-F, IF

Purification

Affinity purified by Protein A

Concentration

1mg/ml

Dilution

WB (WB=1:500-2000), ELISA (ELISA=1:5000-10000), IHC-P (IHC-P=1:400-800), IHC-F (IHC-F=1:400-800), IF (IF=1:100-500)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Unmodified

Storage Conditions

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Gene ID URL

6678

Predicted Cross Reactivity

Human

Curated Selection

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