Phospho-PDHA1 (Ser293) Polyclonal Antibody, Cy3 Conjugated

Product Specifications

Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

Synonyms

PDHA; PDHAD; PHE1A; PDHCE1A; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PDHA1

Gene ID

5160

Swiss Prot

P08559

Modification Site

Ser293

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Mouse, Rat

Target

Phospho-PDHA1 (Ser293)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Cy3

Source

KLH conjugated synthetic phosphopeptide derived from human PDHA1 around the phosphorylation site of Ser293

Applications

Purification

Purified by Protein A.

Excitation Emission

512,550nm/570,615nm

Concentration

1µg/µl

Dilution

WB (1:300-5000)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous across the listed species. This antibody will detect PDHA2, the testis specific form, when phosphorylated at Ser293 in Human, Ser296 in Mouse, and the predicted site of Ser296 in Rat due to sequence homology.