Phospho-PRKAR2A (Ser99) Polyclonal Antibody, PerCP Conjugated

Product Specifications

Background

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Synonyms

PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; PRKAR2A

Gene ID

5576

Swiss Prot

P13861

Modification Site

Ser96

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-PRKAR2A (Ser99)

Clonality

Polyclonal

Isotype

IgG

Conjugation

PerCP

Source

KLH conjugated synthetic phosphopeptide derived from mouse PKA R2 around the phosphorylation site of Ser96

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

482nm/677nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

This phosphorylation site is homologous to that of Ser97 in Rat.

Gene ID URL

5576

Predicted Cross Reactivity

Mouse, Rat

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