Histone H3 (Tri Methyl K27) Antibody, APC Conjugated
Product Specifications
Background
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.
Synonyms
Histone H3, H3 histone family member E pseudogene; H3F3; HIST3H3; Histone H3 3 pseudogene.
Gene ID
3020
Modification Site
Tri Methyl K27
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Histone H3 Tri Methyl K27
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic methyl peptide derived from human Histone H3 around methylation site of Lys27
Applications
WB, FCM
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Methylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
3020
Predicted Cross Reactivity
Rat, Dog, Cow, Sheep, Pig, Rabbit
Curated Selection
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