Phospho-Paxillin (Tyr31) Polyclonal Antibody

Product Specifications

Background

Paxillin is a 64 kDa cytoskeletal adapter protein involved in organisation and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., Actin, Vinculin, Crk) .

Synonyms

PXNphospho Y31; Paired box protein Pax 1; PAX 1; PAX1; Paxillin alpha; PXN; PXN protein; FLJ16691; FLJ23042; PAXI_HUMAN.

Gene ID

5829

Swiss Prot

P49023

Modification Site

Tyr31

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Mouse

Target

Phospho-Paxillin (Tyr31)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31

Applications

ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

A mismatch around the phosphorylation site decreases the antibody's affinity in Mouse/Rat samples

Gene ID URL

5829

Predicted Cross Reactivity

Human, Rat, Cow, Sheep, Horse, Chicken

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