Phospho-Crkl (Tyr251) Polyclonal Antibody, Cy3 Conjugated
Product Specifications
Background
Crkl is a 34kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS dependant manner. Crkl is a substitute of BCR ABL tyrosine kinase. In addition, Crkl has oncogenic potential.
Synonyms
Crk-like protein; CRKL
Gene ID
1399
Swiss Prot
P46109
Modification Site
Tyr251
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-Crkl (Tyr251)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy3
Source
KLH conjugated synthetic phosphopeptide derived from human Crkl around the phosphorylation site of Tyr251 [CA (p-Y) D]
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
512,550nm/570,615nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
1399
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
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