Phospho-DNA PKcs (Thr2609) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Product Specifications
Background
DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs) . DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.
Synonyms
DNA PKcsThr2609; PRKDCphospho T2609; DNA PKcs phospho T2609; p-DNA PKcs phospho T2609; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; Variant; DNAPK; DNAPK catalytic subunit; p350; PRKDC; Protein Kinase DNA Activated Catalytic Polypeptide; XRCC 7; XRCC7; PRKDC_HUMAN.
Gene ID
5591
Modification Site
Thr2609
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse
Target
Phospho-DNA PKcs (Thr2609)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PerCP-Cy5.5
Source
KLH conjugated synthetic phosphopeptide derived from human PRKDC around the phosphorylation site of Thr2609
Applications
IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
482nm/694nm
Concentration
1µg/µl
Dilution
IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5591
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