Phospho-eNOS (Thr495) Polyclonal Antibody, APC-Cy7 Conjugated

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Synonyms

ENOS; ECNOS; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; NOS3

Gene ID

4846

Swiss Prot

P29474

Modification Site

Thr495

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-eNOS (Thr495)

Clonality

Polyclonal

Isotype

IgG

Conjugation

APC-Cy7

Source

KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Thr495

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/780nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

4846

Predicted Cross Reactivity

Rat, Dog, Cow, Sheep, Pig, Rabbit, GuineaPig

Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

abx889809-01 10 nmol

Rat rno-miR-28-5p miRNA Antagomir

Ask

View Details

abx889809-02 20 nmol

Rat rno-miR-28-5p miRNA Antagomir

Ask

View Details

GTR15138087 1 Vial

Lentiviral mouse Cstad shRNA (UAS) - Lentiviral mouse Cstad shRNA (UAS, iRFP) (100)

Ask

View Details

MBS7233721-01 48 Well

Canine ATP-binding cassette sub-family A member 12 (ABCA12) ELISA Kit

Ask

View Details

MBS7233721-02 96 Well

Canine ATP-binding cassette sub-family A member 12 (ABCA12) ELISA Kit

Ask

View Details

MBS7233721-03 5x 96 Well

Canine ATP-binding cassette sub-family A member 12 (ABCA12) ELISA Kit

Ask

View Details