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Phospho-p57 Kip2 (Thr310) Polyclonal Antibody

Product Specifications

Background

Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.

Synonyms

BWS; WBS; p57; BWCR; KIP2; p57Kip2; Cyclin-dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; CDKN1C

Gene ID

1028

Swiss Prot

P49918

Modification Site

Thr310

Cellular Locus

Nucleus

Host

Rabbit

Cross Reactivity

Human, Mouse

Target

Phospho-p57 Kip2 (Thr310)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human p57 Kip2 around the phosphorylation site of Thr310 [EQ (p-T) PR]

Applications

ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

This phosphorylation site is homologous to that of Thr342 in Mouse.

Gene ID URL

1028

Predicted Cross Reactivity

Rat

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