SIAH2 Polyclonal Antibody, Cy5 Conjugated
Product Specifications
Background
Siah2 is a E3 Ubiquitin ligase protein that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targets. Siah2 triggers the ubiquitin-mediated degradation of many proteins, including transcription regulators (e.g. POU2AF1, PML, NCOR1), cell surface receptors (e.g. DCC), antiapoptotic proteins (e.g. BAG1), and proteins involved in synaptic vesicle function in neurons (e.g. SYP) . Siah2 has some overlapping function with SIAH1 but is able to trigger the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 can not.
Synonyms
Seven in Absentia Homolog 2; Ubiquitin Ligase Siah2; SIAH2_HUMAN; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2; Siah-1; Siah-1a; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1.
Gene ID
6478, 6477
Swiss Prot
O43255, Q8IUQ4
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human
Immunogen
201-300/324
Target
SIAH2
Clonality
Polyclonal
Isotype
IgG
Conjugation
Cy5
Source
KLH conjugated synthetic peptide derived from human SIAH2
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
625,650nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
6478, 6477
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Curated Selection
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