Phospho-eNOS (Thr113) Polyclonal Antibody, Biotin Conjugated

Product Specifications

Background

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF) -induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.

Synonyms

ENOS; Nos-3; ecNOS; 2310065A03Rik; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; Nos3

Gene ID

18127

Swiss Prot

P70313

Modification Site

Thr113

Cellular Locus

Cytoplasm, Cell membrane

Host

Rabbit

Cross Reactivity

Human

Target

Phospho-eNOS (Thr113)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Biotin

Source

KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113

Applications

WB, ELISA, IHC-P, IHC-F

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C for 12 months.

Specificity

This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.

Gene ID URL

18127

Predicted Cross Reactivity

Mouse, Rat, Dog, Rabbit

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