Phospho-eNOS (Thr113) Polyclonal Antibody, APC Conjugated
Product Specifications
Background
Synonyms
ENOS; Nos-3; ecNOS; 2310065A03Rik; Nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; NOS type III; NOSIII; Nos3
Gene ID
18127
Swiss Prot
P70313
Modification Site
Thr113
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Human
Target
Phospho-eNOS (Thr113)
Clonality
Polyclonal
Isotype
IgG
Conjugation
APC
Source
KLH conjugated synthetic phosphopeptide derived from mouse eNOS around the phosphorylation site of Ser113
Applications
WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
650nm/660nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Specificity
This phosphorylation site is homologous across the listed species; this phosphorylation site does not exist in human due to a Thr-Ser substitution.
Gene ID URL
18127
Predicted Cross Reactivity
Mouse, Rat, Dog, Rabbit
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