PKC alpha Polyclonal Antibody, PE-Cy5 Conjugated
Product Specifications
Background
Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1) . This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.
Synonyms
AAG6; PKCA; PRKACA; PKC-alpha; Protein kinase C alpha type; PKC-A; PRKCA
Gene ID
5578
Swiss Prot
P17252
Cellular Locus
Cytoplasm, Nucleus, Cell membrane
Host
Rabbit
Cross Reactivity
Human
Immunogen
151-250/672
Target
PKC alpha
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE-Cy5
Source
KLH conjugated synthetic peptide derived from human PKC alpha
Applications
WB
Purification
Purified by Protein A.
Excitation Emission
488nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Unmodified
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
5578
Predicted Cross Reactivity
Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Curated Selection
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