Phospho-ATG1 (Ser556) Polyclonal Antibody, PE-Cy5 Conjugated
Product Specifications
Background
Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
Synonyms
ATG1; ATG1A; UNC51; hATG1; Unc51.1; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1; ULK1; KIAA0722
Gene ID
8408
Swiss Prot
O75385
Modification Site
Ser556
Cellular Locus
Cytoplasm
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat, Rabbit
Target
Phospho-ATG1 (Ser556)
Clonality
Polyclonal
Isotype
IgG
Conjugation
PE-Cy5
Source
KLH conjugated synthetic phosphopeptide derived from human ATG1 around the phosphorylation site of Ser556
Applications
WB, FCM
Purification
Purified by Protein A.
Excitation Emission
488nm/670nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), FCM (1:20-100)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
8408
Predicted Cross Reactivity
Pig, Horse
Curated Selection
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