Phospho-Cardiac Troponin I (Ser23 + Ser24) Polyclonal Antibody, PE Conjugated

Product Specifications

Background

Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Synonyms

ANM; TNT; NEM5; STNT; TNTS; Troponin T, slow skeletal muscle; Slow skeletal muscle troponin T; TNNT1

Gene ID

7138

Swiss Prot

P13805

Modification Site

Ser23 + Ser24

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Phospho-Cardiac Troponin I (Ser23 + Ser24)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Source

KLH conjugated synthetic phosphopeptide derived from human Troponin I around the phosphorylation site of Ser23/24

Applications

WB, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

496,564nm/578nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Specificity

These phosphorylation sites are homologous among the listed cross-reactive species.

Gene ID URL

7138

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