Phospho-Smad3 (Ser423 + Ser425) Polyclonal Antibody, Biotin Conjugated
Product Specifications
Background
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Synonyms
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Gene ID
4088
Swiss Prot
P84022
Modification Site
Ser423 + Ser425
Cellular Locus
Cytoplasm, Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat, Pig
Target
Phospho-Smad3 (Ser423 + Ser425)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Biotin
Source
KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS (p-S) V (p-S) ]
Applications
WB, ELISA, IHC-P, IHC-F
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C for 12 months.
Specificity
These phosphorylation sites are homologous across the species listed.
Gene ID URL
4088
Predicted Cross Reactivity
Dog, Cow, Horse, Chicken
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
RC217436L1 10 µg
Telomerase reverse transcriptase (TERT) (NM_198253) Human Tagged Lenti ORF Clone
Ask
View Details MBS1154170-01 0.02 mg (E-Coli)
Recombinant Acidithiobacillus ferrooxidans Iron--sulfur cluster insertion protein ErpA (erpA)
Ask
View Details MBS1154170-02 0.1 mg (E-Coli)
Recombinant Acidithiobacillus ferrooxidans Iron--sulfur cluster insertion protein ErpA (erpA)
Ask
View Details MBS1154170-03 0.02 mg (Yeast)
Recombinant Acidithiobacillus ferrooxidans Iron--sulfur cluster insertion protein ErpA (erpA)
Ask
View Details