Phospho-B-Raf (Ser602) Polyclonal Antibody

Product Specifications

Background

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Synonyms

NS7; BRAF1; RAFB1; B-RAF1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; BRAF

Gene ID

673

Swiss Prot

P15056

Modification Site

Ser602

Cellular Locus

Cytoplasm, Nucleus, Cell membrane

Host

Rabbit

Cross Reactivity

Human, Mouse, Rat

Target

Phospho-B-Raf (Ser602)

Clonality

Polyclonal

Isotype

IgG

Conjugation

Unconjugated

Source

KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Ser602

Applications

WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Concentration

1µg/µl

Dilution

WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Specificity

Due to the highly conserved nature of this phosphorylated region, this antibody may react with C-Raf when phosphorylated at the Ser494 due to homology.