Phospho-A Raf (Ser299) Polyclonal Antibody, RBITC Conjugated
Product Specifications
Background
The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Synonyms
A RAF phospho S299; p-A RAF phospho S299; A raf 1; A Raf proto oncogene serine/threonine protein kinase; ARAF 1; ARAF; ARaf proto oncogene serine/threonine protein kinase; ARAF1; Oncogene Araf1; Oncogene PKS2; PKS 2; PKS; PKS2; Proto oncogene Pks; RAFA 1; RAFA1; Ras binding protein DA Raf; v raf murine sarcoma 3611 viral oncogene homolog; v raf murine sarcoma 3611 viral oncogene homolog 1; v raf oncogene homolog 1 murine sarcoma 3611 virus; ARAF_HUMAN.
Gene ID
369
Swiss Prot
P10398
Modification Site
Ser299
Cellular Locus
Cytoplasm
Host
Rabbit
Target
Phospho-A Raf (Ser299)
Clonality
Polyclonal
Isotype
IgG
Conjugation
RBITC
Source
KLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser299
Applications
WB, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Excitation Emission
570nm/595nm
Concentration
1µg/µl
Dilution
WB (1:300-5000), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Gene ID URL
369
Predicted Cross Reactivity
Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS9006119 Inquire
Rabbit anti-Caenorhabditis elegans nhr-96 Polyclonal Antibody
Sign In for Pricing
View Details 
MBS1458600-01 0.02 mg (E-Coli)
Recombinant Arabidopsis thaliana LOB domain-containing protein 36 (LBD36)
Sign In for Pricing
View Details