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Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) Polyclonal Antibody, AbBy Fluor™ 647 Conjugated

Selected Variant

bs-3332R-BF647

Size: 100 µL

Product Specifications

Background

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p11 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling.

Synonyms

P85; AGM7; GRB1; IMD36; p85-ALPHA; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1

Gene ID

5295

Swiss Prot

P27986

Modification Site

Tyr467 + Tyr199

Cellular Locus

Cytoplasm

Host

Rabbit

Cross Reactivity

Human, Rat

Target

Phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199)

Clonality

Polyclonal

Isotype

IgG

Conjugation

AbBy Fluor™ 647

Source

KLH conjugated synthetic phosphopeptide derived from human PI3K around the phosphorylation site of p85 alphaTyr467

Applications

WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

Purification

Purified by Protein A.

Excitation Emission

650nm/665nm

Concentration

1µg/µl

Dilution

WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

Buffer

Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Modification

Phosphorylation

Storage Conditions

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Gene ID URL

5295

Predicted Cross Reactivity

Mouse, Dog, Cow, Pig, Horse, Rabbit

Price

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