Phospho-PBK (Thr9) Polyclonal Antibody
Product Specifications
Background
Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Synonyms
SPK; CT84; TOPK; HEL164; Nori-3; Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; MAPKK-like protein kinase; PDZ-binding kinase; Spermatogenesis-related protein kinase; T-LAK cell-originated protein kinase; PBK
Gene ID
55872
Swiss Prot
Q96KB5
Modification Site
Thr9
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-PBK (Thr9)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human PBK/TOPK around the phosphorylation site of Thr9
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
55872
Predicted Cross Reactivity
Human, Rat
Curated Selection
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